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C6638
Sigma
Creatine Phosphokinase from rabbit brain
Type IV, lyophilized powder, 50-150 units/mg protein
| Synonym: | ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase |
| CAS Number: | 9001-15-4 |
| Enzyme Commission (EC) Number: | 2.7.3.2 ( BRENDA | IUBMB ) |
| EC Number: | 232-585-5 |
| MDL number: | MFCD00130876 |
Description
| Analysis Note | Protein determined by biuret. |
| Biochem/physiol Actions | Phosphorylates ADP to form ATP using phosphocreatine as the phosphate donor. |
| Caution | The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution. |
| Unit Definition | One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C. |
| Physical form | Lyophilized powder containing buffer salt as glycine, pH 9.0. |
Properties
| type | Type IV |
| form | lyophilized powder |
| composition | Protein, ~55% |
| foreign activity | ATPase, hexokinase and pyruvate kinase <0.1% |
| lactic dehydrogenase and myokinase <0.05% | |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Inhibitor | C5913, 4-Chloromercuribenzoic acid |
